A laboratory-friendly protocol for freeze-drying sample preparation in ToF-SIMS single-cell imaging
Year: 2025
Type: article
Abstract: ToF-SIMS is a high spatial resolution imaging technique for cellular or subcellular analysis of biological samples. Accurate molecular data in single-cell studies depend on proper cell morphology and chemical integrity, highlighting the importance of sample preparation. In this work, we standardized a more efficient freeze-drying method using standard lab materials and improved the sample preparation process. Our comprehensive freeze-drying protocol for cellular samples, encompassing washing, fixation, and drying steps, facilitates the acquisition of enhanced cellular information and ensures high reproducibility. These improvements are poised to significantly advance single-cell mass spectrometry imaging research.
Source: Frontiers in Chemistry
Authors: Xiujuan Shi, Mingru Liu, Yue Qi, Hongzhe Ma, Zhaoying Wang, Yanhua Chen, Zeper Abliz
Institutions: Minzu University of China, State Ethnic Affairs Commission
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4408239028