Limited Risk of Microbial Contamination During Hypothermic and Normothermic Machine Perfusion of Donor Kidneys
Year: 2025
Type: article
Abstract: BACKGROUND: Both hypothermic machine perfusion (HMP) and normothermic machine perfusion (NMP) are increasingly used for preservation of deceased donor kidneys. However, especially NMP can pose as a risk for microbial contamination of the kidney graft as the 37°C perfusate can act as a breeding ground for microbial contaminants. METHODS: In this study, we investigated the cultures of HMP and NMP perfusates of deceased donor kidneys. RESULTS: Between January 2021 and April 2024, a total of 99 perfusates were examined (73 HMP and 26 NMP perfusates)-. We found that contamination of HMP perfusate was common, occurring in 21 of 73 cultures (29%). Most bacteria originated from the skin. Microbial contamination during NMP was rare, occurring only in 2 of 26 cultures (8%). Microbial contamination during machine perfusion did not lead to infectious complications in the recipients. CONCLUSION: Machine perfusion poses a very limited risk of infection in kidney transplant recipients.
Source: Transplant Infectious Disease
DOI: 10.1111/tid.70019
Institutions: Erasmus MC, Erasmus MC Transplant Institute
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,920
OpenAlex ID: W4408245396