Directed synthesis of N1/N3-histidine modified by 2-hydroxyethylthioethyl and identification in sulfur mustard-exposed plasma
Year: 2025
Type: article
Abstract: Sulfur mustard (HD) alkylates biomolecules such as proteins, generating specific biomarkers. This study employs steric hindrance, electronic effects, and solvent effects through an occupancy-removal strategy to synthesize regioisomers [N1-HETE]-His and [N3-HETE]-His, overcoming isomer separation challenges in conventional methods. Density functional theory (DFT) calculations revealed hexafluoroisopropanol (HFIP)’s critical role in directing HD’s regioselective alkylation: HFIP modulates steric and electronic environments to preferentially target N1 or N3 sites of histidine imidazole rings, with predictions validated experimentally. The method further enables selective detection of the isomers in HD-contaminated plasma via standard addition, advancing absolute quantification. This work not only establishes a precision synthesis platform for biomarkers but also elucidates HFIP’s unique role in imidazole regioselectivity, offering insights for medicinal chemistry and HD toxicology. These findings hold implications for HD exposure tracking, mechanism analysis, clinical diagnostics, and antidote development. Sulfur mustard (HD) is a highly reactive chemical warfare agent capable of alkylating proteins, however, the absolute quantification of HD-exposed biomarkers remains challenging. Here, the authors prepare N1/N3-histidine modified with 2-hydroxyethylthioethyl using precision synthesis strategies and employ them as external standards to identify the HD-exposed plasma.
Source: Communications Chemistry
Authors: Long Wen, Zhibin Shu, Li Pan, Bo Chen, Gang Qu, Shu Geng, Yuntao Yang, Yan Jiang, Shilei Liu
Institution: Sichuan University of Science and Engineering
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,690
OpenAlex ID: W4408247325