Get started
Work

Protocol for single-cell RNA sequencing and spatial transcriptomics of bladder Ewing sarcoma

Year: 2025
Type: article
Abstract: Single-cell RNA sequencing provides a deep understanding of gene expression patterns at the single-cell level, enabling researchers to study cellular heterogeneity and differentiation. Spatial transcriptomics, meanwhile, delivers spatial visualization and quantitative analysis of tissue sections. Here, we introduce a protocol to isolate single cells and obtain high-quality spatial transcriptomics data from bladder Ewing sarcoma tissue. We outline steps for sample collection, single-cell RNA sequencing, and spatial transcriptomics sequencing. For complete details on the use and execution of this protocol, please refer to Mao et al. 1 • Generating highly viable single-cell suspensions from human bladder tissue • Counting and quality control of single-cell suspensions from bladder tissue • Guidelines for preparation of bladder tissue in spatial transcriptomics Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Single-cell RNA sequencing provides a deep understanding of gene expression patterns at the single-cell level, enabling researchers to study cellular heterogeneity and differentiation. Spatial transcriptomics, meanwhile, delivers spatial visualization and quantitative analysis of tissue sections. Here, we introduce a protocol to isolate single cells and obtain high-quality spatial transcriptomics data from bladder Ewing sarcoma tissue. We outline steps for sample collection, single-cell RNA sequencing, and spatial transcriptomics sequencing.
DOI: 10.1016/j.xpro.2025.103687
Authors: Weipu Mao, Houliang Zhang, Jie Ji, Jianpung Wu, Ming Chen, Chao Sun
Language: English
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W4408293896
FWCI: 0.504
Cites: 3
Cited by: 2
Related to: 10