Work

An enzyme‐linked immunosorbent assay ( ELISA )‐based activity assay for AMP ‐activated protein kinase ( AMPK )

Year: 2025

Type: article

Abstract: AMP-activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in modulating metabolism and other cellular processes. Substantial efforts have been made to develop pharmacological modulators of AMPK activity due to their therapeutic potential against various diseases. Measuring AMPK activity in vitro is a fundamental step for testing AMPK activators and inhibitors. Here, we report an enzyme-linked immunosorbent assay (ELISA)-based AMPK activity assay with simple steps and high sensitivity. This assay offers a robust, in-house alternative to the traditional radioactive methods and other approaches that rely on specialized reagents or commercial kits.

Source: FEBS Open Bio

DOI: 10.1002/2211-5463.70017

Authors: Trezze P. Nguyen, Shangze Lyu, Yang Liu

Institutions: The University of Texas Health Science Center at Houston, The University of Texas MD Anderson Cancer Center

Language: English

Topic: Metabolism, Diabetes, and Cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,210