Development of Fluorescence-Based Method for Dopamine Determination Using o-Phthaldialdehyde and 3-Mercaptopropyltriethoxysilane
Year: 2025
Type: article
Abstract: Nanomaterials and sensors play an important role in modern technologies, including medical diagnostics and biochemical research. This work presents the possibility of using o-Phthaldialdehyde (OPA) in combination with 3-mercaptopropyltriethoxysilane (MPTES) to develop a dopamine-responsive sensor. During the experiment, these materials were used at different pH and ratios to determine the optimal parameters for obtaining high fluorescence intensity of the reaction product. The data obtained demonstrate a linear relationship between the fluorescence response (λex/λem = 340/460 nm) of OPA/MPTES and dopamine concentration in the range of 0.1–3.0 µM at a pH of 8, and the detection limit was 8.7 nM. The obtained results confirm the potential of OPA/MPTES as a sensing component for the detection of dopamine.
Source: Sensors
DOI: 10.3390/s25061729
Authors: Valeriia Sliesarenko, Marijana Krstić, Urban Bren, Aleksandra Lobnik
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,137
Awards: Understanding the mechanisms and prevention of aggregation of biopharmaceutic proteins, G1692205387, G1732733373, BBMRI, Multi-omic analysis of NFkB-mediated signaling in multiple sclerosis, G2894027807, Chemical Carcinogenesis and Neurodegeneration: Molecular-Level Mechanistic Impact of Microwave Irradiation, G3240721, MultiPart: A Novel Approach for Improving Health and Well-being in Anthropogenically Perturbed Environments Based on Multi-Participatory Research, Optical Chemical/Bio Sensor Systems (OPTISENS), G3689878489, G4427557255, Unveiling the Analgesic Potential of Herkinorin: A Comprehensive Approach to Designing Safer Opioids, G4824746457, Design of novel (nano)material properties & Applications, G6059675823, G6065173288, G6143541106, Separation Processes and Product Design, Library of opioid binding patterns in correlation with their adverse side effects, Coronaviral therapeutic target mutagenesis platform for communal treatment evaluation and the development of pan-coronavirus inhibitors, G798108181, Development of Electrochemical Biosensor for Detection of Methylmercury, G887191007
OpenAlex ID: W4408338940