Work

Quantitative imaging of species-specific lipid transport in mammalian cells - endocytosis inhibitors

Year: 2025

Type: dataset

Abstract: To test the predominant role of the non-vesicular route for retrograde transport in U-2 OS cells, we inhibited vesicular trafficking using Brefeldin A, which blocks COPI coat formation at Golgi membranes and Wortmannin, which broadly affects endosome formation and trafficking and quantified transport of SM(Y), PE(18:1/Y), PC(Y:16:0), PC(Y/20:4) using the lipid imaging pipeline.

DOI: 10.6019/s-biad1695

Authors: Juan M. Iglesias-Artola, Kristin Böhlig, Kai Schuhmann, Katelyn C. Cook, Hjoerdis Mathilda Lennartz, Milena Schuhmacher, Pavel Barahtjan, Cristina Jiménez López, Radek Šachl, Vannuruswamy Garikapati, Karina Pombo-Garcia, Annett Lohmann, Petra Riegerová, Martin Hof, Björn Drobot, Andrej Shevchenko, Alf Honigmann, André Nadler

Institutions: Max Planck Institute of Molecular Cell Biology and Genetics, École Polytechnique Fédérale de Lausanne, Czech Academy of Sciences, Czech Academy of Sciences, Heyrovsky Institute, Helmholtz-Zentrum Dresden-Rossendorf

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4408347930

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