Detection and characterization of the Dezidougou virus (genus Negevirus) in mosquitoes (Ochlerotatus caspius) collected in the Republic of Sakha (Yakutia)
Year: 2025
Type: article
Abstract: INTRODUCTION: Monitoring and research on arthropod-borne microorganisms is important. Recently, with the development of next-generation sequencing methods, many previously unknown viruses have been identified in insects. AIM OF THE STUDY: , including determination of its complete nucleotide sequence, phylogenetic and virological characteristics. MATERIALS AND METHODS: ) cell culture. Electron microscopy was performed using a JEM 1400 electron microscope. Nucleotide sequence screening was performed by NGS on a high-throughput sequencer MiSeq, Illumina (USA). Full genome nucleotide sequence was determined by Sanger sequencing. Phylogenetic analysis was performed using GenBank database, using Vector NTI Advance 11 and MEGA 11 programs. RESULTS: The virus isolated from mosquitoes replicated efficiently in C6/36 cells, causing their death. However, it did not replicate in the mammalian cell cultures used. The isolated virus did not cause pathologic manifestations in suckling mice when infected intracerebrally. Electron microscopic examination of the purified virus-containing suspension showed the presence of spherical viral particles with a diameter of 45‒55 nm. The results of full genome sequencing identified it as belonging to Dezidougou virus, first isolated in Côte d'Ivoire. The nucleotide sequence of the genome of Yakutsk 2023 strain of Dezidougou virus was deposited in GenBank (PP975071.1). CONCLUSION: Dezidougou virus of genus Negevirus was isolated and characterized for the first time in the Russian Federation. Further studies on the prevalence of negeviruses, their virological features, potential importance for public health and their impact on vector competence of vectors are important and promising.
Source: Problems of Virology
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4408360615