Work

Synthesis of Selectively 13 C/ 2 H/ 15 N‐ Labeled Arginine to Probe Protein Conformation and Interaction by NMR Spectroscopy

Year: 2025

Type: article

Abstract: The charged arginine side chain is unique in determining many innate properties of proteins, contributing to stability and interaction surfaces, and directing allosteric regulation and enzymatic catalysis. NMR experiments can be used to reveal these processes at the molecular level, but it often requires selective insertion of carbon-13, nitrogen-15, and deuterium at defined atomic positions. We introduce a method to endow arginine residues with defined isotope patterns, combining synthetic organic chemistry and cell-based protein overexpression. The resulting proteins feature NMR active spin systems with optimized relaxation pathways leading to simplified NMR spectra with a sensitive response to changes in the chemical environment of the nuclei observed.

Source: Chemistry - A European Journal

DOI: 10.1002/chem.202500408

Authors: Darja I. Rohden, Giorgia Toscano, Paul Schanda, Roman J. Lichtenecker

Institutions: University of Vienna, Institute of Science and Technology Austria

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,640

Funder: Austrian Science Fund