Work

Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent

Year: 2025

Type: article

Abstract: This study evaluated the optomechanical and structural properties of individual macroscopic layers in swine skin tissues treated with a nontoxic optical clearing agent. The clearing agent was prepared by dissolving 2,2'-thiodiethanol in a phosphate-buffered solution and applied for up to 6 days. Prolonged clearing increased both the total and unscattered transmittance. Peaks associated with deoxygenated hemoglobin, oxygenated hemoglobin, and water showed marked reduction with extended clearing times. Histopathological examination revealed no significant structural alterations. A clearing time of approximately 4-5 days is recommended for optimal imaging, as it minimizes changes in mechanical properties. These findings support the use of optical clearing in deep tissue imaging, particularly when preserving macroscopic mechanical properties is essential, despite the inherent limitation of sample opacity.

Source: Journal of Biophotonics

DOI: 10.1002/jbio.70007

Authors: Noemi Correa, Minji Suh, Abdul Mohizin, Jinu Sudhakaran, Kee Sung Lee, Jung Kyung Kim

Institutions: Kookmin University, KIST Europe

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,370