Work

Amelioration of Glutamate-induced Toxicity by a New Thyrotropin-releasing Hormone (TRH) Analogue PYR- l -(2,5-Dibromo)-His- l -ProNH 2

Year: 2025

Type: article

Abstract: Background: Glutamate has been implicated in the pathophysiology of central nervous system diseases, including stroke. Purpose: ; NP-2376] against glutamate-induced injury model were investigated. Methods: Cortical neurons isolated from neonatal rats were used to evaluate the effects of the NP-2376. Cortical neurons were pre-treated with NP-2376 (6, 12 and 24 h) prior to glutamate (15 mM) exposure. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assay, and oxidative stress by chloromethyl-2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) and glutathione assays. Results: NP-2376 protected against glutamate-induced cortical neuron death and oxidative stress in a dose-dependent manner. Conclusions: This study demonstrates the neuroprotective potential of TRH analogue NP-2376 against glutamate-induced toxicity, which is attributed to a decrease in oxidative stress.

Source: Annals of Neurosciences

DOI: 10.1177/09727531241305505

Authors: Mallikarjuna R. Sunkara, Jitendra N. Singh, C. L. Meena, A. B. Pant, Rahul Jain, Shyam S. Sharma

Institutions: National Institute of Pharmaceutical Education and Research, National Institute of Pharmaceutical Education and Research, Indian Institute of Toxicology Research

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $395