Work

Efficient Particle Capture and Release Method for DNA Library Preparation on Microfluidics

Year: 2025

Type: article

Abstract: To address the issues of agglomeration during magnetic particle capture and the incomplete release of these particles during reuse in microfluidic chips for library preparation, a microchamber was utilized to enhance the dispersion area for magnetic particle capture. Additionally, the release of magnetic particles was achieved through the synergistic action of flow field and magnetic field. The simulation results indicated that as the inlet flow velocity varied from 0.02 m/s to 0.16 m/s and the magnet spacing ranged from 1.2 mm to 1.8 mm, the coverage of magnetic particles in the microchamber increased from 17.29% to 63.59%. Meanwhile, the magnetic particle capture rate decreased from 100% to 35.2%. These processes were further validated through experimental methods. During the release process, the trajectory of magnetic particles under the synergistic effect of flow field and magnetic field aligned with expectations. The captured magnetic particles were released from the microchamber within 12 s, achieving a release rate of 100%.

Source: Micromachines

DOI: 10.3390/mi16030332

Authors: Zihan Song, Yihui Wu, Fengfeng Shu, Xiao Lv, Junyu Dong, Huan Li

Institutions: Chinese Academy of Sciences, Changchun Institute of Optics, Fine Mechanics and Physics, University of Chinese Academy of Sciences

Language: English

Topic: Microfluidic and Bio-sensing Technologies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,534

Funder: National Key Research and Development Program of China