Work

Supercritical CO2 Extraction of Bioactive Compounds from Corn Grains (Zea mays L., Hybrid Pri-15-7-16) with Metabolomic Profiling and Confocal Laser Microscopy

Year: 2025

Type: article

Abstract: This study aimed to optimize supercritical CO2 extraction conditions, analyze bioactive compounds, and visualize their distribution in corn grains (Zea mays L., hybrid Pri-15-7-16). The optimal extraction conditions were identified as a pressure of 200 bar and a temperature of 55 °C, yielding 2.2 mg/g of bioactive compounds. The distribution of autofluorescent compounds within corn grain tissues was visualized using confocal laser scanning microscopy. Image analysis showed that the pericarp and aleurone layer cell walls were rich in autofluorescent compounds, while the endosperm cell walls exhibited low autofluorescence. Metabolomic analysis, combining high-performance liquid chromatography and mass spectrometry, identified 44 compounds in the extracts, including 30 polyphenolic compounds from subgroups such as polyphenolic acids, flavones, flavan-3-ols, flavonols, and anthocyanidins as well as 14 compounds from other chemical groups, including amino acids and fatty acids.

Source: Plants

DOI: 10.3390/plants14060913

Authors: Mayya P. Razgonova, Pavel A. Shinkaruk, Anastasiia A. Maksimenko, Anna B. Podvolotskaya, Liudmila A. Tekutyeva

Institutions: Far Eastern Federal University, N.I. Vavilov Research Institute of Plant Industry

Language: English

Topic: Phytochemicals and Antioxidant Activities

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,258