A Novel Peptide-Based Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Neutralizing Antibodies Against NADC30-like PRRSV GP5 Protein
Year: 2025
Type: article
Abstract: Porcine reproductive and respiratory syndrome (PRRS) is a pig respiratory disease threating the global swine industry. It is necessary for the effective diagnostic detection of antibody including neutralizing antibody against porcine reproductive and respiratory syndrome virus (PRRSV), especially the currently prevalent NADC30-like PRRSV in China. In this study, we prepared three monoclonal antibodies (mAbs) against NADC30-like PRRSV glycoprotein 5 (GP5) protein, and identified two corresponding precise epitopes (155WR156 and 196QWGRP200). In neutralization test, 196QWGRP200 recognizing GP5 mAbs (11E6 and 12D1) exhibited obvious neutralizing activity, whereas the 155WR156 recognizing mAb (3A8) showed low neutralizing activity. Based on the two antigenic peptides, the peptide-based ELISA was developed to detect antibodies against PRRSV, presenting high specificity, sensitivity and repeatability. The concordance rate of the peptide-based ELISA and commercial IDEXX PRRSV X3 Ab ELISA in detection of 81 clinical samples was 82.7%. In conclusion, the GP5 peptide-based ELISA can be used for the detection of neutralizing antibodies against NADC30-like PRRSV, providing a rapid and reliable method for monitoring PRRSV infection.
DOI: 10.3390/ijms26062619
Authors: Shaohua Sun, Kaili Zhang, Jiajia Zhang, Pingping Zhang, Ping He, Dafu Deng, Sen Jiang, Wanglong Zheng, Nanhua Chen, Jianfa Bai, Jianzhong Zhu
Institutions: Yangzhou University, Kansas State University
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,499
Awards: G4589639268, G5534499278, G7679892080
OpenAlex ID: W4408457396