Inhibitory effect of standardized Kaempferia parviflora extract on sarcopenia by improving protein metabolism pathways in aged C57BL/6J mice
Year: 2025
Type: article
Abstract: Background: extract (KPE) on sarcopenia and elucidate its underlying molecular mechanisms. Methods: To assess the inhibitory effects of KPE on sarcopenia, 18-month-old C57BL/6J mice were employed as an aged model. The mice were orally administered 100 and 200 mg/kg KPE daily for 8 weeks. Results: KPE improved age-reduced grip strength and exercise endurance. It not only increased hindlimb muscle weight, volume, and myofiber cross-sectional area but also decreased abdominal fat volume and adipose tissue weight. KPE downregulated the mRNA expression of tumor necrosis factor alpha and interleukin-6 by inactivating nuclear factor kappa B protein expression and increasing the mRNA expression of anti-oxidant enzymes. Moreover, it significantly reduced the mRNA expression of muscle ring finger-1 and muscle atrophy F-box for proteolysis and stimulated the mammalian target of rapamycin pathway for protein translation by stimulating the phosphatidylinositol 3-kinase/Akt pathway. KPE also increased the relative mitochondrial DNA content by upregulating peroxisome proliferator-activated receptor gamma coactivator 1 alpha, nuclear respiratory factor 1, and mitochondrial transcription factor A mRNA expression. Conclusion: This study's findings demonstrate that KPE improves age-induced sarcopenia by regulating protein turnover and mitochondrial homeostasis. Therefore, KPE is potentially useful as a functional ingredient that inhibits or decelerates sarcopenia.
Institution: Yonsei University
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4408465156