Work

Protocol for preparing mouse hippocampal slices for ex vivo recordings of the temporoammonic pathway

Year: 2025

Type: article

Abstract: The temporoammonic pathway (TAP), the direct input from the entorhinal cortex to the hippocampus, is an important model structure for investigating synaptic plasticity. Here, we present a protocol for preparing TAP containing ex vivo slices from mice and conducting extracellular recordings of TAP inputs. We describe the specific slicing plane, an angled horizontal slice preparation, along with anatomical hallmarks for the identification of stimulation and recording electrode placement. We additionally describe techniques to verify TAP electrical stimulation via pharmacological and optogenetic manipulations. For complete details on the use and execution of this protocol, please refer to Keary et al. 1 • Instructions for stereotaxic viral injection into the entorhinal cortex in mice • Details for preparing brain slices preserving the temporoammonic pathway • Procedures for recording field potentials of temporoammonic pathway stimulation • Techniques for optogenetic and pharmacological confirmation of temporoammonic stimulation Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The temporoammonic pathway (TAP), the direct input from the entorhinal cortex to the hippocampus, is an important model structure for investigating synaptic plasticity. Here, we present a protocol for preparing TAP containing ex vivo slices from mice and conducting extracellular recordings of TAP inputs. We describe the specific slicing plane, an angled horizontal slice preparation, along with anatomical hallmarks for the identification of stimulation and recording electrode placement. We additionally describe techniques to verify TAP electrical stimulation via pharmacological and optogenetic manipulations.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103698

Authors: Kevin M. Keary, Zheng Li

Institutions: National Institutes of Health, National Institute of Mental Health

Language: English

Topic: Memory and Neural Mechanisms

Subfield: Cognitive Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: National Institutes of Health, National Institute of Mental Health