Work

Evaluation of toxicity and anti-amylase activity of 7, 8 dihydroxy coumarin (Daphnetin), a novel α-amylase blocker in vitro and in vivo

Year: 2025

Type: article

Abstract: Management of postprandial blood glucose is a measure in regulating the blood sugar concentration. This paper evaluates the anti-amylase property of a natural compound 7,8 dihydroxycoumarin (daphnetin) both in vitro and in vivo . The inhibitory effect of daphnetin was evaluated against salivary (HSA) and pancreatic α-amylases (PPA) and α-glucosidase (AG) in vitro . Enzyme kinetics studies revealed that the inhibition of HSA, PPA and AG by daphnetin was competitive in nature. Pretreatment with daphnetin was found to inhibit the amylases in vivo as confirmed by the oral starch tolerance test (OSTT). No adverse effect of daphnetin was observed on serum markers and organs like liver, kidney and small intestine. The impact of the inhibitory effect of daphnetin in in vivo was comparable to the positive control, acarbose in all aspects. • 7,8 dihydroxycoumarin or Daphnetin inhibits α-amylases and α-glucosidase in vitro . • The inhibition of the enzymes by daphnetin was competitive in nature. • Oral starch tolerance test confirmed anti-amylase property of daphnetin in vivo. • Administration of daphnetin for 7 days had no adverse effect on the mice. • The results were comparable to acarbose, the positive control.

Source: Toxicology Reports

DOI: 10.1016/j.toxrep.2025.101991