Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model
Year: 2025
Type: article
Abstract: When skin is exposed to ultraviolet B (UVB) radiation, damaged keratinocytes release double-stranded RNA to trigger an inflammatory response. Here, we present a protocol to induce epidermal-specific Zfp750 gene-deficient mice and establish a UVB-induced skin inflammation model. We detail procedures for detecting gene expressions in the epidermis by quantitative reverse-transcription PCR (RT-qPCR) and analyzing changes in skin immune cell subsets after UVB radiation using flow cytometry. For complete details on the use and execution of this protocol, please refer to Liu et al. 1 • Instructions for inducing epidermal-specific gene deletion in K14-CreER tam mice • Steps on establishing a UVB-irradiated skin inflammation model • Guidance on epidermal isolation for gene expression analysis • Instructions for preparing skin cell suspension for flow cytometry staining Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. When skin is exposed to ultraviolet B (UVB) radiation, damaged keratinocytes release double-stranded RNA to trigger an inflammatory response. Here, we present a protocol to induce epidermal-specific Zfp750 gene-deficient mice and establish a UVB-induced skin inflammation model. We detail procedures for detecting gene expressions in the epidermis by quantitative reverse-transcription PCR (RT-qPCR) and analyzing changes in skin immune cell subsets after UVB radiation using flow cytometry.
Source: STAR Protocols
Authors: Ye Liu, George L. Sen
Institution: University of California San Diego
Language: English
Topic: Skin Protection and Aging
Subfield: Dermatology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W4408495252