Work

Generation of TP53 knock out induced pluripotent stem cell using CRISPR/Cas9

Year: 2025

Type: article

Abstract: The TP53 gene is an important tumor suppressor gene. Through CRISPR/Cas9 technology, we have established a TP53 gene knockout cell line in iPSCs (SIIBRi001-A). This cell line maintains normal stem cell-like morphology, karyotype, expresses markers of pluripotency, and is capable of generating teratomas in immunodeficient mice. Quantitative analysis of pluripotency gene expression remains normal. This cell line can be utilized for studying the mechanisms underlying tumorigenesis.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2025.103699

Authors: Haonan Zhou, Yaonan Liu, Qu Chen, Ke Huang, Pei-Gen Ren

Institutions: Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,360