Work

Model‐based aberration corrected microscopy inside a glass tube

Year: 2025

Type: article

Abstract: Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In nonstandard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably impairing high-resolution imaging. Correcting such large aberrations using standard adaptive optics can be challenging: existing solutions are either not suited for strong aberrations, or require extensive feedback measurements, consequently taking a significant portion of the photon budget. We demonstrate that it is possible to precompute the corrections needed for high-resolution imaging inside a glass tube based on a priori information only. Our ray-tracing-based method achieved over an order of magnitude increase in image contrast without the need for a feedback signal.

Source: Journal of Microscopy

DOI: 10.1111/jmi.13402

Authors: Daniël W. S. Cox, Tom Knop, Ivo M. Vellekoop

Institution: University of Twente

Language: English

Topic: Adaptive optics and wavefront sensing

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,760

Funders: European Commission, Horizon 2020 Framework Programme, H2020 European Research Council