In Vitro Comparison of the Viability and Proliferation of Human Gingival Fibroblasts and Osteoblast-like MG-63 Cells on Three Different Temporary Cements Used in Dental Implants
Year: 2025
Type: article
Abstract: Background: Regarding retained cement acts as an idiopathic cause for early implant loss, cement selection with the least toxicity in the peri-implant hard and soft tissues is important. The present study aimed to evaluate the cytotoxicity of three different types of temporary cements and titanium cemented with each cement after direct exposure to human gingival fibroblasts (HGF) and MG-63 osteoblast-like cells.Methods: In this in vitro study, zinc oxide-eugenol (ZOE), eugenol-free zinc oxide (ZONE), and resin (R) cements prepared in cylindrical forms with a similar dimension were used. Each cement was placed on titanium disks to prepare cemented titanium samples. MTT assay was applied at 24- and 72-hour, and 7-day intervals to evaluate cytotoxicity.Results: All the cements decreased cell viability in both cell lines significantly. None of the cements exhibited cellular viability percentages higher than the minimum percentage (70%) required for biocompatibility. The cemented titanium disks’ cytotoxicity was not significantly different from the cement-only samples (P>0.05). There were no significant differences in the sensitivity of MG-63 osteoblast-like cells and HGF to the cements evaluated. Conclusions: The composition of the cement had a significant role in the host cell response. This study showed that dental cements could cause tissue toxicity in the gingiva and bones, finally affecting implant survival.
Source: Frontiers in Dentistry
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $215
OpenAlex ID: W4408499742