Work

Thermodynamic perspectives into DNA stability and information encoding in the human genome

Year: 2025

Type: article

Abstract: The perpetuation of species depends on two critical factors at the DNA level: the encoding of information essential for survival and adaptation, and the stability of DNA to preserve this information. Focusing on the latter, this study posits and confirms that local interactions within DNA are sufficient to explain the observed Chargaff’s symmetries, providing a thermodynamic foundation for genome stability. Our analyses show that encoding genetic information incurs an energetic cost, with an effective energy expenditure of approximately one-tenth of kT per base when comparing exonic sequences to intronic and intergenic regions. Furthermore, pathogenic mutations tend to drive segments toward lower effective energy states, with pronounced effects observed in disease-associated mutations. By evaluating the effective energy of DNA sequences, this framework offers valuable insights into the interplay between physical principles, information encoding, and mutation dynamics. DNA physical stability is crucial for preserving genetic information. This study shows that local energy interactions alone explain Chargaff’s symmetries, linking genome organization to fundamental thermodynamic principles. Furthermore, encoding genetic information incurs an energetic cost, influencing mutation dynamics and shaping evolutionary processes.

Source: Communications Physics

DOI: 10.1038/s42005-025-02025-0

Authors: Piero Fariselli, Amos Maritan

Institutions: University of Turin, University of Padua

Language: English

Topic: Fractal and DNA sequence analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,990

Funders: European Commission, Università degli Studi di Padova, Università degli Studi di Torino