Smooth muscle cell Piezo1 depletion results in impaired contractile properties in murine small bowel
Year: 2025
Type: article
Abstract: Piezo1 is a mechanosensitive cation channel expressed in the intestine's smooth muscle cells (SMCs), where its role has not been investigated. We used diverse methods to determine whether Piezo1 in murine SMCs is required to mediate myogenic contractility at rest and following stretch. Using an inducible system to deplete Piezo1 from SMCs led to impaired growth and delayed motility. The Piezo1 deficient SMCs had decreased contractile strength that was asynchronous, dysrhythmic, and less frequent compared to control samples at baseline and following stretch. Moreover, ablation of SMC Piezo1 led to thinning of the muscularis, changes in the cellular composition of the epithelial layer, and a decline in pacemaker cells within the muscularis, among other changes. Our data suggest that Piezo1 in the SMCs of the small bowel is required for modulating myogenic contractile strength and rhythmicity and maintaining cell types needed to promote motility.
Source: Communications Biology
Authors: Geoanna M. Bautista, Yingjie Du, Michael J. Matthews, Allison M. Flores, Nicole R. Kushnir, Nicolle K. Sweeney, Nam Phuong N. Nguyen, Elmira Tokhtaeva, R. S. Solorzano-Vargas, Michael Lewis, Matthias Stelzner, Ximin He, James C. Y. Dunn, Martin G. Martin
Language: English
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $4,190
Funders: U.S. Department of Health and Human Services, California Institute for Regenerative Medicine, American Academy of Pediatrics, Hartwell Foundation, Children's Discovery Institute, National Institutes of Health, University of California, Los Angeles, University of California, Davis, California State University, Northridge, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Advancing Translational Sciences
Awards: G1113868446, G1276652605, G1361649777, G156123175, CSUN-UCLA Bridges to Stem Cell Research, G1878893643, Neurogenin3 and Intestinal Failure, G2126705276, G257993041, G285791796, G3644367333, Gastroenterology, G4823198893, G557103581, G5623947192, CURE: Digestive Diseases Research Center, G6015208799, G7006020220, G762012121, Piezo-1 & 2’s role in murine intestinal muscularis cells of the SIP syncytium, UC Davis Clinical and Translational Science Center, Institutional Career Development Core (KL2), COngenital Diarrhea and Enteropathy (PediCODE) Consortium and BioRepository
OpenAlex ID: W4408527537