Work

Flow-Induced Shear Stress Combined with Microtopography Inhibits the Differentiation of Neuro-2a Cells

Year: 2025

Type: article

Abstract: Considering that neurological injuries cannot typically self-recover, there is a need to develop new methods to study neuronal outgrowth in a controllable manner in vitro. In this study, a precise flow-controlled microfluidic system featuring custom-designed chambers that integrate laser-microstructured polyethylene terephthalate (PET) substrates comprising microgrooves (MGs) was developed to investigate the combined effect of shear stress and topography on Neuro-2a (N2a) cells' behavior. The MGs were positioned parallel to the flow direction and the response of N2a cells was evaluated in terms of growth and differentiation. Our results demonstrate that flow-induced shear stress could inhibit the differentiation of N2a cells. This microfluidic system could potentially be used as a new model system to study the impact of shear stress on cell differentiation.

Source: Micromachines

DOI: 10.3390/mi16030341

Authors: Eleftheria Babaliari, Paraskevi Kavatzikidou, Dionysios Xydias, Sotiris Psilodimitrakopoulos, Anthi Ranella, Emmanuel Stratakis

Institutions: FORTH Institute of Electronic Structure and Laser, Foundation for Research and Technology Hellas, University of Crete

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,534

OpenAlex ID: W4408529662

FWCI: 2.092

Cites: 69

Cited by: 5

Related to: 10