Work

Anti-inflammatory and Xanthine Oxidase Inhibitor of Melicope Ptelefolia Leaves - A Promising Source of Gout Treatment

Year: 2025

Type: article

Abstract: Background The leaves and rhizomes of Melicope pteleifolia are utilized for their therapeutic properties, being applied to treat various ailments such as scabies, wounds, ulcers, sore throats, rheumatism, eczema, dermatitis, bruises, and snake bites. The goal of this work is to investigate the anti-inflammatory and xanthine oxidase inhibitory activities as well as the isolation of bioactive compounds of extracts and fractions from Melicope ptelefolia . Methods The dried plants were extracted exhaustedly with 96% ethanol, the solvent was evaporated, and the resulting extracts (MPE) were partitioned successively with hexane, ethyl acetate, and methanol through flash chromatography to obtain three sub-fractions (MPH, MPEA, and MPM). The macrophage cell line RAW264.7 was used to assess the anti-inflammatory activity via inhibition of secretion of mediator (NO and TNF-α). The DPPH, ABTS, RP assay, and xanthine oxidase inhibition were applied to investigate the antioxidant and reducing uric acid in vitro, respectively. Structural chemicals were identified by NMR methods. Results All the extracts manifested anti-inflammatory and antioxidant activities. MPEA fraction showed a better antioxidant and NO inhibitory effects than that of MPE and MPH, while MPH fraction was stronger than those of MPE, MPEA, and MPM in the TNF-α inhibition experiment. From the most active fraction (MPEA), eight compounds (1-8) were isolated and identified by column chromatography. Of which, quercetin (3), quercitrin (7), kaempferol (2), and Luteolin 7 glucoside (8) were the potential compounds for anti-inflammation and suppressing activity of xanthine oxidase. Conclusions Melicope ptelefolia exhibited significant anti-inflammatory, antioxidant, and xanthine oxidase inhibitory properties, showing that it was a promising source for developing medications targeting inflammation and elevated uric acid levels. Its bioactive compounds are potentially used in pharmaceuticals to treat conditions like gout and other inflammatory illnesses.

Source: Natural Product Communications

DOI: 10.1177/1934578x251328859

Authors: Le Xuan Binh, An Nguyen Minh Tran, Dung Tien Le

Institutions: Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Industrial University of Ho Chi Minh City

Language: English

Topic: Gout, Hyperuricemia, Uric Acid

Subfield: Nephrology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,650