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Constant Enzymatic in situ Production of H2O2 for an Unspecific Peroxygenase by an l– Amino Acid Oxidase

Year: 2025

Type: article

Abstract: Unspecific Peroxygenases have been the subject of a lot of research in the recent past as they offer opportunities for green hydroxylation reactions using hydrogen peroxide (H2O2) as donor. A striking problem of UPOs is their low stability against their own co‐substrate H2O2. In order to prevent the deactivation, we developed an enzyme cascade with an l‐amino acid oxidase (LAAO) to produce hydrogen peroxide in situ at a constant low concentration, aiming for much higher lifespan and therefore longer working time of the enzyme. This way, we are able to convert a variety of substrates with conversion rates up to 95%.

Source: Advanced Synthesis & Catalysis

DOI: 10.1002/adsc.202500105

Authors: Simon Last, Tobias Heinks, Niklas Dietz, Simon Koopmeiners, Gabriele Fischer von Mollard, Martin J. Weissenborn, Jan von Langermann

Institutions: Otto-von-Guericke-Universität Magdeburg, Delft University of Technology, Martin Luther University Halle-Wittenberg, Bielefeld University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,650

OpenAlex ID: W4408587071

FWCI: 1.828

Cites: 30

Cited by: 7

Related to: 10