Work

Elucidating the molecular mechanism of myofibrillar protein-luteolin affecting lipid accumulation and oxidative stress in HepG2 cells

Year: 2025

Type: article

Abstract: The luteolin (Lut) binding with myofibrillar protein (MP) can enhance the functionality of proteins, having the potential to ameliorate obesity in the form of dietary intervention. Our previous research unveiled the in vitro cell viability and antioxidant properties of the MP-derived and Lut-containing multicomponent peptides (MDLPs). This study aimed to further investigate the effect of MDLPs intervention and downstream effects on lipid metabolism using integrated physiological and multi-omics approaches. The results demonstrated that MDLPs significantly ameliorated steatosis and systemic oxidative stress in HepG2 cells induced by a high-fat (HF) reagent. Combining metabolomic and transcriptomic analysis indicated that peroxisome proliferator-activated receptor (PPAR), forkhead box protein O (FoxO), and amino acids metabolism signaling pathways played pivotal roles in response to the MDLPs intervention, primarily through upregulating PPARα, PPARγ, FOXO1, and Perilipin 4 (PLIN4). The abundance of potential disease biomarkers (4-hydroxynonenal and hydroxylinoleic acid) were enhanced. The outcomes of this research provide the theoretical basis for the formulation of novel meat products with anti-obesity activity.

Source: Food Science and Human Wellness

DOI: 10.26599/fshw.2025.9250523

Authors: Zhenyang Wu, Rui Zheng, Xue Zhao, Xinglian Xu

Institution: Nanjing Agricultural University

Language: English

Topic: Protein Hydrolysis and Bioactive Peptides

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: National Natural Science Foundation of China, Priority Academic Program Development of Jiangsu Higher Education Institutions, Earmarked Fund for China Agriculture Research System