Work

A Comparative Analysis of Transfected and Integrated Auxin Reporter Systems Reveals Sensitivity Advantages in Protoplast Transient Expression Assays

Year: 2024

Type: article

Abstract: Reporter-gene activation studies using transient transformation of protoplasts are a powerful tool for the investigation of transcriptional regulation in plants. Here, we perform a comparative analysis of reporter-gene activation sensitivity using an integrated versus a co-transfected reporter-gene construct in Arabidopsis seedling mesophyll protoplasts. The DR5 synthetic auxin-responsive promoter was used to assay the response to auxin treatment and over-expression of activator Auxin Response Factors. We show that sensitivity, as measured by the fold-change in fluorescent-protein reporter-gene expression, is significantly increased by using a co-transfected reporter-gene construct.

Source: PubMed

DOI: 10.17912/micropub.biology.001481

Authors: Joseph S. Taylor, Eric A. Villaseñor, James Rashkovsky, Jaime Simson, R. Clay Wright, Bastiaan O. R. Bargmann

Institution: Virginia Tech

Language: English

Topic: Plant Molecular Biology Research

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institute of General Medical Sciences

Awards: Collaborative Research: Hormonal control of stamen filament growth, G892216102