Functional and structural profiling of circulation via genetically encoded modular fluorescent probes.
Year: 2025
Type: preprint
Abstract: Sustained labeling of fluids is crucial for their investigation in animal models. Here, we introduce a mouse line (Alb-mSc-ST), where blood and interstitial fluid are labeled with the red fluorescent protein mScarlet and SpyTag. The SpyTag-SpyCatcher technology is exploited to monitor circulating fluid properties by biosensors or detect blood-brain barrier disruption. This approach represents a valuable tool for studying vascular structure, permeability and microenvironment in body organs in vivo .
Authors: Marta Vittani, Ashley Bomin Lee, Xiaowen Wang, Yuichi Hiraoka, Daichi Sasaki, Ayumu Konno, Philip Gade Knak, Peter Kusk, Masaki Nagao, Julien Courtin, Muhammad Fadhli Putranto, Yusuke Nasu, Saki Tsuno, Ken Ueda, Yuri Osuga, Takashi Tsuboi, Alma Piganiol, Thomas Bienvenu, Miho Terunuma, Hirozaku Hirai, Maiken Nedergaard, Kohichi Tanaka, Antonios Asiminas, Hajime Hirase
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4408608076