Work

Combined selective plane illumination microscopy (SPIM) and full-field optical coherence tomography (FF-OCT) for in vivo imaging

Year: 2025

Type: article

Abstract: Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context, which is essential for the comprehensive analysis of biological samples. Here, we present a high-resolution, multimodal imaging system that integrates SPIM with full-field optical coherence tomography (FF-OCT), without requiring modifications to the existing SPIM setup. Both SPIM and FF-OCT offer low phototoxicity and intrinsic optical sectioning, making them well-suited for in vivo imaging. Their shared detection path enables seamless and efficient co-registration of fluorescence and structural data. We demonstrate the functionality of this combined system by performing in vivo imaging of zebrafish larvae.

Source: Optics Continuum

DOI: 10.1364/optcon.551385

Authors: Rui Ma, Olga Lyraki, Daniel Wehner, Jochen Guck

Language: English

Topic: Optical Coherence Tomography Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,372

Funders: Deutsche Forschungsgemeinschaft, Max-Planck-Gesellschaft

Awards: Biophysikalische Methoden für die Quantifizierung von Mechanik, Formen und Kräften, G2143627703, G2470032517, SFB 1540: Erforschung der Mechanik des Gehirns (EBM): Verständnis, Engineering und Nutzung mechanischer Eigenschaften und Signale in der Entwicklung, Physiologie und Pathologie des zentralen Nervensystems