Simultaneous combination of subcritical water extraction and enzyme-assisted extraction for protein recovery from lime peels. Characterization of protein hydrolysates
Year: 2025
Type: article
Abstract: Lime peels are citrus industry residues constituting a great source of bioactive compounds. Reusing these residues contributes to sustainability and reduces the negative environmental impact of food waste removal. In this work, environmentally friendly strategies based on green techniques were developed for the efficient extraction of proteins from lime peels. Two techniques, non-employed before with this aim, were employed: subcritical water extraction (SWE) and enzyme-assisted extraction (EAE), as well as their simultaneous combination. SWE with Viscozyme Wheat HT as polysaccharide enzyme enabled a high extraction yield (95 %) in a short analysis time. Proteases Thermolysin and Alcalase were evaluated to release peptides with antioxidant, antihypertensive, and antimicrobial activities from protein extracts. Most active hydrolysates were analysed by UHPLC-Q-TOF-MS/MS enabling to identify 30 peptides along with 44 phenolics, and other bioactive compounds. Some of these compounds are associated to the observed bioactivities, highlighting the potential contribution of the ACE-related antihypertensive peptide FDAVGVK. • SWE applied for the first time to recover proteins from lime peels. • The simultaneous combination of SWE and EAE increased protein extraction yield. • 30 peptides identified in the most active protein hydrolysates by UHPLC-Q-TOF-MS/MS. • 44 polyphenols and other compounds identified in protein extracts and hydrolysates. • Peptide FDAVGVK could be responsible for the ACE related antihypertensive capacity.
Source: Food Chemistry
Institution: Universidad de Alcalá
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,930
Award: G7214409398
OpenAlex ID: W4408627199