Work

Positive Effects of Seabuckthorn oil on Scopolamine Induced PC12 Cell Damage and APP/PS1 Mice

Year: 2025

Type: article

Abstract: Objective Cognitive dysfunction is common in neurodegenerative diseases. Sea buckthorn oil (SBO) has anti-inflammatory and antioxidant properties. So, we want to investigate the effect of SBO on reducing scopolamine-induced oxidative stress, inflammation, and cholinergic changes in PC12 cells, and its impact on ameliorating cognitive dysfunction in APP/SP1 mice. The purpose of this study is to prove that SBO has a positive effect on cognitive dysfunction. Method In vitro, the effect of SBO on scopolamine-induced PC12 cells damage is evaluated using the 3- (4,5-dimethylthiazol-2-yl) −2,5-diphenyltetrazolium bromide (MTT) method. Levels of various biochemical markers were also examined by enzyme-linked immunosorbent assay (ELISA), including acetylcholinesterase (AChE), and acetylcholine (ACh). As well as examining the inflammatory factors: interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), and interleukin-6 (IL-6). The oxidative stress of cell was detected using reactive oxygen species (ROS) staining. In addition, we detected catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), which are indicators of oxidative stress (OS) in vitro experiments. Moreover, the detection of Nrf2/HO-1 molecules and inflammatory cytokines released by reverse transcription-polymerase chain reaction (RT-PCR) and western blot. In vivo, the memory behavior is evaluated using the Morris water maze(MWM) and step-down test. Result SBO showed a significant reduction of AChE activities and increased the viability of scopolamine-treated PC12 cells. Meanwhile, SBO increases the level of GSH-Px, SOD, CAT activities, and ACh, and decreases the level of TNF-α, IL-1β, and IL-6. Moreover, increasing the expression of the Nrf2/HO-1 pathway. SBO has shortened the swimming time to the platform, and increased the exploration time of the target quadrant and the number of times it crosses the platform. Meanwhile, it reduced the number of errors in the step-down test. Conclusion SBO has a protective effect against scopolamine-induced PC12 cell injury and a favorable impact on improving cognitive impairment in APP/PS1 mice. Therefore, SBO may have clinical applications in the treatment of cognitive impairment.

Source: Natural Product Communications

DOI: 10.1177/1934578x251329127