Regulation of WNT16 in bone may involve upstream enhancers within CPED1
Year: 2025
Type: article
Abstract: WNT16 stands up as an essential gene for bone homeostasis. Here, we present new evidence of the functional role of a particular region within WNT16. Performing 4 C chromatin conformation analysis in three osteoblast-related cells (the human fetal osteoblast hFOB 1.19 cell line, Saos 2 osteosarcoma cell line and mesenchymal Stem Cells -MSC-), we identify physical interactions between the proximal part of WNT16 intron 2, shown here to be an active promoter in Saos 2 osteosarcoma cells, and several putative regulatory regions within CPED1. Analysis of previously published RNA-seq data from hFOB cells disclosed low expression of a region located downstream of this promoter. Our results suggest a novel regulatory mechanism of WNT16 in bone, mediated by physical interaction with various enhancer regions within CPED1.
Source: Scientific Reports
Institutions: Instituto de Salud Carlos III, Centre for Biomedical Network Research on Rare Diseases, Institut de Recerca Sant Joan de Déu, Institut de Biomedicina de la Universitat de Barcelona, Universitat de Barcelona, European Molecular Biology Laboratory, Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable, Hospital del Mar Research Institute
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Award: G1568148067
OpenAlex ID: W4408667698