Work

Rosmarinic Acid Alleviated Cyclophosphamide-Induced Gonadal Toxicity in Adult Male Rats

Year: 2025

Type: article

Abstract: Background/Aim: This study aimed to investigate the potential protective effects of rosmarinic acid (RA) against cyclophosphamide (CP)-induced gonadal toxicity in male Wistar Albino rats. Specifically, the research focused on the modulation of apoptotic pathways, with an emphasis on Bax protein expression, and utilized bioinformatic analyses to elucidate the key molecular mechanisms and signaling pathways underlying the observed effects. Materials and Methods: The experimental design consisted of four groups: Control (administered saline), RA (administered rosmarinic acid), CP (administered cyclophosphamide), and RA+CP (administered a combination of rosmarinic acid and cyclophosphamide). Following a 14-day treatment period, body weight, serum malondialdehyde (MDA) levels, and Bax protein expression in testicular tissue were evaluated. Additionally, a protein-protein interaction (PPI) network influenced by RA and CP was constructed using STITCH and subsequently analyzed in Cytoscape. Functional enrichment analysis was performed to identify key molecular pathways associated with Bax regulation, with an emphasis on clusters exhibiting significant associations (p

Source: Cukurova Anestezi ve Cerrahi Bilimler Dergisi

DOI: 10.36516/jocass.1619585

Authors: Firat Sahin, Engin Deveci, Fırat Aşır, Merve Gulsen Bal Albayrak, Ebru Gökalp Özkorkmaz

Institutions: Dicle University, Kocaeli Üniversitesi, Ankara Yıldırım Beyazıt University

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Dicle Üniversitesi