Injectable Magnetic-Nanozyme Based Thermosensitive Hydrogel for Multimodal DLBCL Therapy
Year: 2025
Type: article
Abstract: Diffuse large B-cell lymphoma (DLBCL), accounting for 31% of non-Hodgkin lymphomas, remains recalcitrant to conventional therapies due to chemoresistance, metastatic progression, and immunosuppressive microenvironments. We report a novel injectable Fe3O4@DMSA@Pt@PLGA-PEG-PLGA hydrogel system integrating magnetothermal therapy (MHT), chemodynamic therapy (CDT), and immunomodulation. Under alternating magnetic fields (AMF), the system achieves rapid therapeutic hyperthermia (50 °C within 7 min) while activating pH/temperature-dual responsive peroxidase (POD) -like activity in Fe3O4@DMSA@Pt nanoparticles. Catalytic efficiency under tumor-mimetic conditions was significantly higher than Fe3O4@DMSA controls, generating elevated reactive oxygen species (ROS). Flow cytometry revealed 75.9% apoptotic cell death in A20 lymphoma cells at 50 °C, significantly surpassing CDT alone (24.5%). Importantly, this dual mechanism induced immunogenic cell death (ICD) characterized by 4.1-fold CRT externalization, 68% HMGB1 nuclear depletion, and 40.74 nM ATP secretion. This triggered robust dendritic cell maturation (92% CD86+/CD80+ DCs comparable to LPS controls) and T cell activation (16.9% CD25+/CD69+ ratio, 130-fold baseline). Our findings validate the therapeutic potential of magnetothermal-chemodynamic synergy for DLBCL treatment, paving the way for innovative multi-mechanism therapeutic strategies against DLBCL with potential clinical translation prospects.
Source: Gels
DOI: 10.3390/gels11030218
Authors: Min Yan, Jingcui Peng, Haoan Wu, Ming Ma, Yu Zhang
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,534
Awards: G1116222579, G1132155481, G1337856307, G140145392, G1403535049, G1681342209, G1704914635, G1748125952, G2050233153, G2291019199, G2361838037, G2794319767, G2838301289, G2857109069, G3323122899, G3378032544, G3395212270, G3478116217, G3568346096, G3624436111, G3729814037, G3792070678, G3993529064, G4057359616, G4300758506, G4366999025, G4369994034, G4462981160, G4560832362, G4739162398, G4750010636, G4869906953, G5035777060, G5157957658, G5299396795, G5792903113, G6446958842, G6490087079, G6570275115, G6655204934, G7209965508, G8070109036, G8078570750, G8444985465, G8498285169, G8588972544, G8596750268, G8689523670, G8748496173, G8789993870, G8866475079, G8912393668
OpenAlex ID: W4408693289