Work

An in silico and in vitro approach for understanding CDK2 expression pattern and prognostic implications in breast cancer

Year: 2025

Type: article

Abstract: CDK2 (Cyclin-Dependent Kinase 2) is a Ser/Thr kinase that regulates cell cycle progression by promoting the G1/S transition and initiating DNA synthesis through its interaction with cyclins E and A. Unchecked cell growth, DNA damage, and protein deterioration are caused by an abnormally high level of CDK2. When it does so, it responds in excess, which triggers unchecked cell proliferation, which is what happens in breast cancer The study's primary objective is to explore CDK2's expression profile, functional role, and prognostic importance in the development of breast cancer In this study, we evaluated CDK2's expression profile, prognostic implications and interactions with other proteins to better understand how CDK2 influences the etiology of breast cancer. A range of computational techniques, including UALCAN, TIMER2.0, GEPIA, STRING, DISCO, ENRICHR, and Docking, were utilized Breast cancer patients had highly regulated CDK2 levels. Utilizing cytohubba, the most significant 10 hub genes of the web were identified. The regulation of heterochromatin formation and cellular response to nitric acid are substantially enriched in the KEGG pathway analysis and gene ontology GO. Association with the p53 pathway was revealed by the KEGG pathway investigation. Adapalene strongly bound to protein CDK2 in a 3D schematic, with the lowest binding energy being −10.87 kcal/mol Collectively, the study shows that CDK2 promotes tumor growth and that blocking it in addition to conventional therapies will significantly enhance the medical outcomes of breast cancer patients. • The enzyme cyclin-dependent kinase 2 has roles in cell cycle, and DNA damage and protein deterioration. • CDK2 inhibition blocks the expansion of endocrine-resistant breast carcinoma cells. • CDK2 expression is frequently very high in malignant tumors. • One effective anticancer strategy for breast cancer is the in-vitro suppression of CDK2 with the drug Adapalene. • Targeting CDK2 in conjunction with other therapeutic drugs in a combination approach has enormous potential to improve treatment outcomes for patients with breast carcinoma.

Source: Results in Chemistry

DOI: 10.1016/j.rechem.2025.102207

Authors: Fatima Manhas, Shazia Sofi, Asma Jan, Nusrat Jan, Manzoor Ahmad Mir

Institution: University of Kashmir

Language: English

Topic: Breast Cancer Treatment Studies

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,870