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Nanoliposome-encapsulated MAGE peptides: A promising melanoma vaccine to activate human dendritic cells

Year: 2025

Type: article

Abstract: Melanoma immunotherapy with MAGE-A/B-targeted vaccines faces challenges in achieving robust clinical responses. This study aimed to develop and evaluate a nanoliposome-encapsulated melanoma vaccine delivering MAGE-A and MAGE-B antigenic peptides to stimulate human dendritic cells (DCs). MAGE-A and MAGE-B peptide sequences were selected based on bioinformatics analysis. Nanoliposomes encapsulating these peptides were synthesized and characterized, and their ability to stimulate interleukin (IL)-1 and IL-2 expression in human DCs in vitro was assessed. The synthesized nanoliposomes were spherical, averaged 110 nm in diameter, and efficiently encapsulated approximately 90 % of the peptides. In vitro experiments showed that exposure of DCs to the nanoliposomes resulted in increased IL-1 and IL-2 gene expression, with a maximum effect at a concentration of 240 μg/mL after 48 h. Nanoliposome encapsulation of MAGE-A/B peptides effectively delivers antigen to DCs and stimulates cytokine production in vitro. This nanoliposome-based vaccine shows promise as a potential melanoma immunotherapy strategy and warrants further in vivo investigation.

Source: Results in Chemistry

DOI: 10.1016/j.rechem.2025.102218