Year: 2025
Type: article
Abstract: Organic selenium, a bioavailable form of the essential trace element selenium, holds significant potential for improving human health through dietary supplements and functional foods. However, low bioconversion efficiency has primarily limited the biosynthesis of organic selenium compounds. Here, we focused on enhancing the biosynthesis of organic selenium by optimising the expression and activity of two key enzymes, SenB and SenC, involved in the conversion process. We compared several expression systems, including fusion expression and dual-promoter approaches, and optimised reaction conditions such as temperature, pH and incubation time. Our results showed that mutations of SenC more than doubled enzyme activity, resulting in a corresponding rise in the intermediate SeP. Notably, the fusion expression of SenB and SenC exhibited the highest conversion rate of organic selenium, achieving over 95% under optimal conditions. Our findings provide a basis for organic selenium production through microbial biotechnology.
Source: Microbial Biotechnology
Authors: Kailin Shao, Xiaobin Yu, Yan Zhao, Ying Zhang, Xiaobo Liu
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $4,260
Awards: G2905297771, G5500266725
OpenAlex ID: W4408750740