An optimized protocol for generating fibrotic murine precision-cut lung slices
Year: 2025
Type: article
Abstract: Precision-cut lung slices (PCLSs) offer significant advantages over both in vivo animal models and in vitro cell models, making them invaluable tools in respiratory research. Here, we present a protocol for generating fibrotic mouse PCLSs (mPCLSs), which offer the same benefits as human PCLSs but bypass issues of human tissue availability and related ethical concerns while also enhancing experimental homogeneity and reproducibility. This protocol details the steps for bleomycin (BLM)-induced pulmonary fibrosis, PCLS generation, visualization, and downstream analysis. For complete details on the use and execution of this protocol, please refer to Barbayianni et al. 1 • Instructions for inducing pulmonary fibrosis in mice using bleomycin (BLM) • Procedures for generating precision-cut lung slices (PCLSs) • Steps for histological staining, RNA extraction, and viability assays Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Precision-cut lung slices (PCLSs) offer significant advantages over both in vivo animal models and in vitro cell models, making them invaluable tools in respiratory research. Here, we present a protocol for generating fibrotic mouse PCLSs (mPCLSs), which offer the same benefits as human PCLSs but bypass issues of human tissue availability and related ethical concerns while also enhancing experimental homogeneity and reproducibility. This protocol details the steps for bleomycin (BLM)-induced pulmonary fibrosis, PCLS generation, visualization, and downstream analysis.
Source: STAR Protocols
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W4408805482