Isolation of Exstraintestinal Pathogenic E.coli from ostcholecystectomic Samples and their Antimicrobial profile in Georgia
Year: 2025
Type: article
Study design: Observational Study
Abstract: Since 2009, we have been studying infections caused by pathogenic E. coli in Georgia (STEC infection and HUS syndrome). The work discusses the role of pathogenic E. coli and EBL strains in the development of extraintestinal abdominal pathology - chronic calculous cholecystitis. The frequency of highly resistant, extended-spectrum beta-lactamase-producing E. Coli - (ESBL) strains has increased worldwide. To determine the specific role of E. Coli in the development of chronic calculous cholecystitis, bacteriological analysis of 130 postoperative gallbladder tissues was performed and the sensitivity of the isolated cultures to antibiotics is currently being studied. Genome analysis of ESBL strains (MISEQ —Illumina Platform) (NCDC) is currently underway. Gallbladder tissue from 130 patients was examined. 28 cultures (22%) were isolated, including EE.COli-9 cases (32%); 6 of them were ESBL strains. The dominance of E.Coli-strains was revealed compared to other pathogens. We studied the sensitivity of the isolated E.Coli strains to antibiotics. ESBL-producing E.Coli strains were resistant to penicillin and cephalosporin antibiotics, and resistance to ciprofloxacin and imipenem was also established. Thus, in the bacteriological study of post-cholecystectomy material, in various gallbladder pathologies, the presence of ESBL-producing E.Coli strains indicates the circulation of highly resistant E.Coli strains in Georgia.
Authors: Elene Pachkoria, Elza Vashakidze, Tamar Megrelishvili, Lia Tevzadze
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4408806380