Work

Polyphenol-Rich Snack Consumption during Endurance Exercise Training Improves Nitric Oxide Bioavailability but does not Improve Exercise Performance in Male Cyclists: A Randomised Controlled Trial

Year: 2025

Type: article

Study designs: Randomized Controlled Trial, Clinical Trial

Abstract: Background Antioxidants and nitric oxide (NO) precursors may improve endurance exercise performance by reducing oxidative stress and increasing NO production. Almonds, dried grapes, and cranberries (AGC) are good sources of antioxidants and NO precursors. Objectives To determine whether AGC consumption improved physiological responses and endurance cycling time-trial performance in response to training. Methods After 1 wk of light training (LT), 96 male recreationally trained cyclists consumed 125 g of AGC or control (CON: isocaloric oat bar) daily during 2 wk of heavy training (HT) and a 2-wk taper (T). At the end of LT, HT, and T, endurance exercise performance (5-min cycling time-trial; 5CTT), NO bioavailability (plasma and urine nitrate and nitrite), oxidative stress [plasma F2-isoprostanes (F 2 -Isop)], muscle damage (creatine kinase) and subjective measures of wellbeing were assessed, as well as physiological responses during exercise at 70% maximal aerobic power output. Results Compared to LT, 5CTT performance was impaired at HT ( d = –0.27, P = 0.01) and improved at T ( d = 0.79, P < 0.001), with no difference between treatments ( P > 0.81). Compared with CON, during submaximal exercise at 70%, maximal aerobic power output AGC demonstrated higher oxygen consumption (HT: d = 0.46; T: d = 0.38, P < 0.001) and lower respiratory exchange ratio (HT: d = –0.61; T: d = –0.23, P < 0.032). At HT, urine F 2 -Isop was higher compared with LT ( d = 0.21, P = 0.036), but plasma F 2 -Isop was lower ( d = –0.22, P = 0.008 ) , with no difference between treatments. At HT, AGC had higher subjective energy concentrations ( d = 0.21, P = 0.02) and urinary nitrite ( d = 0.23, P = 0.03) compared with CON and higher creatine kinase ( d = 0.24, P = 0.02) and less fatigue ( d = –0.20; P = 0.05) at T. Conclusions Although not beneficial for 5CTT performance or exercise efficiency, AGC increases fat oxidation during exercise, NO bioavailability, and subjective energy concentrations, which may confer benefits for health and wellbeing. This trial was registered at www.anzctr.org.au as ACTRN12618000360213.

Source: Current Developments in Nutrition

DOI: 10.1016/j.cdnut.2025.106006