Dezocine Suppresses Myocardial Cell Apoptosis in Rats with Myocardial Ischemia-Reperfusion Injury via TLR4/NF-κB Signaling Pathway
Year: 2025
Type: article
Abstract: Background Myocardial ischemia-reperfusion (MI/R) injury is a leading cause of myocardial damage, characterized by apoptosis and impaired cardiac function. Dezocine, a mixed opioid receptor agonist/antagonist, has shown potential in mitigating MI/R injury, but its underlying mechanisms remain unclear. Objectives This study aimed to evaluate the effects of dezocine on myocardial cell apoptosis in rats with MI/R injury, focusing on the TLR4/NF-κB signaling pathway. Materials and Methods Thirty-six Sprague-Dawley rats were randomly divided into three groups: sham, model, and dezocine ( n = 12 each). The sham group underwent thoracotomy without MI/R injury. The model group received normal saline prior to MI/R injury induction, while the dezocine group was administered dezocine. Toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB) p65 expression were analyzed using immunohistochemistry. Western blotting quantified Bax and Caspase-3 protein levels while quantitative polymerase chain reaction assessed their mRNA expression. Cell apoptosis was evaluated via terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Results TLR4 and NF-κB p65 expression were significantly elevated in the model and dezocine groups compared to the sham group but notably lower in the dezocine group versus the model group. Bax and Caspase-3 protein and mRNA levels followed a similar trend. TUNEL assay results demonstrated higher apoptosis rates in the model and dezocine groups compared to the sham group, with the dezocine group showing a marked reduction in apoptosis compared to the model group ( p < 0.05). Conclusion Dezocine suppresses the TLR4/NF-κB pathway, reducing myocardial apoptosis in MI/R injury, suggesting its potential as a therapeutic agent in mitigating cardiac damage.
Source: Pharmacognosy Magazine
Authors: Mingyan He, Ru Liu, Yalin Li, Qi Zhang
Institutions: Hunan Normal University, Hunan Provincial People's Hospital
Language: English
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $1,600
OpenAlex ID: W4408817149