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Integrating Scatac-seq With Imaging Modalities Via Magnetic-based Deterministic Barcoding

Year: 2024

Type: conference-paper

Abstract: Single cell approaches are essential for unveiling cell heterogeneity and biological mechanisms [1].Microwells represent a standard tool for imaging, and they can facilitate cell isolation prior to single cell assay for transposase-accessible chromatin with sequencing (scATAC-seq).To directly correlate chromatin accessibility profiles with cell or organelle morphology, both datasets need to be linked using barcodes, sequences that can spatially associate the scATAC-seq profile to the corresponding image.Current methods for barcoding like droplet printing are laborious and time consuming[2], while previous microfluidic-based deterministic barcoding strategies have focused on tissue sections [3,4], and are not readily adaptable to microwells due to the heightened risk of cross-talk.

DOI: 10.70477/uggc2675

Authors: Anna Fomitcheva Khartchenko, Trinh Lam, Amy Herr

Institutions: University of California, Berkeley, Chan Zuckerberg Biohub San Francisco

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W4408818325

FWCI:

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