Rapid Lamp-based Colorimetric Mrsa Detection Using a Microfluidic System Integrated With Accelerated Electrophoretic Nucleic Acids Purification
Year: 2024
Type: conference-paper
Abstract: We demonstrated a fully integrated sample-to-answer microfluidic system for methicillin-resistant staphylococcus aureus (MRSA) detection.This system can rapidly and accurately detect MRSA from just few microliters of serum sample.To be specific, it integrates multiple functions on-a-chip, including pathogen lysis, electrophoretic purification and loop-mediated isothermal amplification (LAMP) of DNA.The colorimetric detection result can be directly determined by naked eye at limit-of-detection as low as 100 CFU per reaction.
DOI: 10.70477/ctks6980
Authors: Yung Ching Lee, Yang Bu, Sheng Ni, Yuze Liu, Anni Hu, Levent Yobas
Institution: Hong Kong University of Science and Technology
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
OpenAlex ID: W4408818352