Estimating the Accuracy of Intraperitoneal Drug Administration in Adult Zebrafish
Year: 2025
Type: article
Abstract: Intraperitoneal injections are commonly used for systemic drug administration in various laboratory animals, including rodents and zebrafish ( Danio rerio ) . Although these fish have rapidly gained popularity in biomedical research, the accuracy of intraperitoneal drug delivery (due to potential leakage from the injection site) has not been previously evaluated in zebrafish. To investigate this issue, we injected adult zebrafish with 10 µL/g of a dye (1% Evans Blue) intraperitoneally and estimated dye leakage by measuring the optical density of water where the fish were kept for 20 min past injection. Overall, we found that intraperitoneal injections resulted in an estimated approximately 10% immediate leakage and approximately 0.01% secondary leakage (of the injected dye volume), confirming the relative accuracy of intraperitoneal injection as a reliable drug delivery method in adult zebrafish.
Authors: Kirill V Apukhtin, Maria M Kotova, David S Galstyan, Adam Michael Stewart, Murilo S de Abreu, Allan V Kalueff
Institutions: St Petersburg University, Federal Almazov North-West Medical Research Centre, Sirius University of Science and Technology, Ministry of Health of the Russian Federation, Institute of Experimental Medicine, Universidade Federal de Ciências da Saúde de Porto Alegre, Western Caspian University, Xi’an Jiaotong-Liverpool University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4408827013