Year: 2024
Type: conference-paper
Abstract: Mapping the proteome of low-input samples and single cells is crucial to elucidate mechanisms in disease states such as cancer.However, almost all previous efforts to miniaturize existing sample preparation protocols for low-input protein samples[1] are based on one pot in-solution methods, where lysis reagents need to be compatible with liquid chromatography-mass spectrometry (LC-MS).In contrast, clean-up methods enable washing steps and as such are compatible with stronger detergents, which provide more efficient cell lysis and protein solubilization [2].Here, we present for the first time different steps taken towards an integrated microfluidic approach for clean-up-based proteomic sample preparation for low-input samples.
DOI: 10.70477/nkbh8077
Authors: Aurélie Mohrbacher, Gemma Jacobs, Jolien Breukers, Iene Rutten, An Staes, Marcel Bühler, Francis Impens, Kris Gevaert, Jeroen Lammertyn
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
OpenAlex ID: W4408828138