Work

Integrated Microfluidic Platform for Single Organoid Culture and Secreted Extracellular Vesicle Isolation

Year: 2024

Type: conference-paper

Abstract: In this abstract, we present a versatile microfluidic platform enabling the isolation of extracellular vesicles (EVs) secreted by a single organoid.Our automated device combines both the on-chip culture of a single organoid and an integrated micropump downstream allowing the continuous secretion sampling.Leveraging an immunomagnetic capture protocol, we achieve selective and efficient EVs isolation from whole secretions.This work is paving the way for an integrated companion test assay based on EVs secretion monitoring from organoids.

DOI: 10.70477/acxm5845

Authors: Marie Hut, Flora Clement, Frederic Bottausci, Josiane Denis, Manuel Alessio, François Boizot, Nadia Cherradi, Myriam Cubizolles, Fabrice Navarro, Yves Fouillet, Vincent Agache

Institutions: Commissariat à l'Énergie Atomique et aux Énergies Alternatives, CEA Grenoble, Laboratoire d'Électronique des Technologies de l'Information, Inserm

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W4408828230

FWCI: 1.866

Cites:

Cited by: 1

Related to: 10