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Concurrent Detection of Protein and Mirna at the Single Extracellular Vesicle Level Using a Digital Dual Crispr-cas Assay

Year: 2024

Type: conference-paper

Abstract: Simultaneous detection of protein and RNA at the single extracellular vesicle (EV) level is necessitated for precise disease profiling due to the heterogeneity and scarcity of tumor-derived EVs.Therefore, the multiplexed, easy-to-operate, and cost-effective method for single EV analysis is highly desired.In this study, a digital dual CRISPR-Cas-powered Single EV Analysis (ddSEA) system was proposed to enable the concurrent detection of surface protein and inner miRNA of EVs at the single-molecule level.

DOI: 10.70477/ihas6505

Authors: Xun Xu, Yuanyue Zhang, Muxue Wang, Youchun Xu

Institution: Tsinghua University

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4408828247

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