Work

Antibiofilm activity of Clitoria ternatea flowers anthocyanin fraction against biofilm-forming oral bacteria

Year: 2025

Type: article

Abstract: This study investigated the antibiofilm effects of Clitoria ternatea flowers anthocyanin fraction (AF) on Streptococcus mutans, Actinomyces viscosus, and Aggregatibacter actinomycetemcomitans. AF was obtained using column chromatography, and liquid chromatography-mass spectrometry was employed for its characterization and identification. The crystal violet assay and scanning electron microscopy analysis revealed significant inhibition of early biofilm formation and destruction of preformed biofilms after AF treatment (0.94-15 mg ml-1). Antiadhesion assay on acrylic teeth demonstrated that AF effectively hampered sucrose dependent and independent attachment. Importantly, growth curve and pH drop assays showed that AF inhibited pH reduction for all bacteria tested without hindering bacterial growth. Furthermore, the tetrazolium-based cytotoxicity assay indicated no toxicity towards normal human gingival fibroblasts at 0.78-12.5 mg ml-1. These findings suggest C. ternatea anthocyanins are promising antibiofilm agents for oral biofilm control, acting during both initial formation and on mature biofilms.

Source: FEMS Microbiology Letters

DOI: 10.1093/femsle/fnaf035

Authors: Allimalar Sathiaseelan, Keang Peng Song, Hock Siew Tan, Wee Sim Choo

Institution: Monash University Malaysia

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,297