Work

3′- O -β-Glucosylation of nucleoside analogues using a promiscuous bacterial glycosyltransferase

Year: 2025

Type: article

Abstract: sp. AVP053U2 is investigated toward a panel of both natural and clinically relevant purine and pyrimidine nucleoside analogues. AvpGT demonstrates broad substrate promiscuity, with glycosylation observed by HILIC-MS for 15 of 21 nucleosides tested. Of these, 12 nucleosides were successfully glycosylated on ≥25 μmol scale in 39-91% isolated yields, including four current therapeutics.

Source: RSC Chemical Biology

DOI: 10.1039/d5cb00026b

Authors: Jonathan P. Dolan, Tessa Keenan, Aisling Ní Cheallaigh, Martin A. Fascione, Gavin J. Miller

Institutions: Keele University, University of York

Language: English

Topic: Carbohydrate Chemistry and Synthesis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,962